Journal: Cell Death Discovery

Article Title: CLC3 regulates V-ATPase to enhance lysosomal degradation and cisplatin resistance in cervical cancer cells

doi: 10.1038/s41420-025-02876-0

Figure Lengend Snippet: A Relative CLC3 mRNA expression levels in various CVC cell lines (SiHa, MS751, HeLa, ME180, Hela229, CaSKi) compared to normal cervical epithelial cells (NCCs). * P < 0.05, ** P < 0.001. vs. NCCs. B Western blot analysis showing CLC3 protein expression in NCCs and different CVC cell lines (SiHa, MS751, HeLa, ME180, Hela229, CaSKi), with GAPDH as a loading control. * P < 0.05, ** P < 0.001. vs. NCCs. C CLC3 mRNA expression in CVC cell lines (SiHa, MS751, HeLa, ME180, Hela229, and CaSKi) following CLC3 overexpression (CLC3 High ) or knockdown (CLC3 Low ) compared to control. * P < 0.05, ** P < 0.001. vs. Control. D Western blot analysis of CLC3 protein levels in CLC3 High , CLC3 Low , and control cells across the same cell lines, with quantification shown in the accompanying graphs. * P < 0.05, ** P < 0.001. vs. Control. E IC50 values for cisplatin, paclitaxel, and 5-FU in CLC3 High , CLC3 Low , and control groups in SiHa and CaSKi cells, showing CLC3 modulation’s effect on chemoresistance. * P < 0.05 vs. Control.

Article Snippet: CVC cell lines (SiHa, MS751, HeLa, ME180, Hela229, CaSKi) and normal cervical epithelial cells (NCCs) were obtained from the American Type Culture Collection (ATCC) and maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin at 37 °C in a humidified atmosphere with 5% CO2.

Techniques: Expressing, Western Blot, Control, Over Expression, Knockdown

Journal: PeerJ

Article Title: Morphometric analysis of a triple negative breast cancer cell line in hydrogel and monolayer culture environments

doi: 10.7717/peerj.4340

Figure Lengend Snippet: (A) HCC70 cells cultured on rigid substratum showed the characteristic morphology described by the vendor (ATCC; Manassas, VA). Cells showed horizontal spreading, and close association with neighboring cells. (B) HCC70 cells formed spheroidal aggregates when cultured in the hydrogel. The data are representative of two independent experiments. Scale bar = 50 µm.

Article Snippet: The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers): The human breast cancer cell line HCC70 (ATCC ®CRL 2315TM; Lot #58033362; RRID: CVCL_1270) was obtained from the American Type Culture Collection (ATCC; Manassas, VA).

Techniques: Cell Culture

Journal: PeerJ

Article Title: Morphometric analysis of a triple negative breast cancer cell line in hydrogel and monolayer culture environments

doi: 10.7717/peerj.4340

Figure Lengend Snippet: The spatial arrangements of actin-based structures and cell nuclei in monolayer and hydrogel cultures of HCC70 cells were visualized using confocal microscopy by labeling with fluorescent probes—Alexa Fluor ® 488 Phalloidin (green) for F-actin and Hoechst 33342 (blue) for DNA. (A–C) During confocal projections of labeled cells, monolayer cells appeared flat. The cells also appeared to be situated far from each other. (D–F) In hydrogel environment, cells formed spheroids and the cells appeared to adhere closely to one another. Scale bar = 10 µm.

Article Snippet: The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers): The human breast cancer cell line HCC70 (ATCC ®CRL 2315TM; Lot #58033362; RRID: CVCL_1270) was obtained from the American Type Culture Collection (ATCC; Manassas, VA).

Techniques: Confocal Microscopy, Labeling

Journal: PeerJ

Article Title: Morphometric analysis of a triple negative breast cancer cell line in hydrogel and monolayer culture environments

doi: 10.7717/peerj.4340

Figure Lengend Snippet: Quantitative analysis of TEM images captured from monolayer and hydrogel cultures of HCC70 cells. TEM images were captured from four blocks for each condition—monolayer and hydrogel. The number of mitochondria, autophagic vacuoles and intercellular junctions were counted from six images obtained from each block. The numbers shown in the table represent the sum of counts obtained from six images.

Article Snippet: The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers): The human breast cancer cell line HCC70 (ATCC ®CRL 2315TM; Lot #58033362; RRID: CVCL_1270) was obtained from the American Type Culture Collection (ATCC; Manassas, VA).

Techniques: Blocking Assay

Journal: PeerJ

Article Title: Morphometric analysis of a triple negative breast cancer cell line in hydrogel and monolayer culture environments

doi: 10.7717/peerj.4340

Figure Lengend Snippet: HCC70 cells grew adjacent to each other in hydrogel and comprised several mitochondria (A–D,‘m’), autophagic vacuoles (A, C,‘av’) and intercellular junctions (A, B,‘*’). The data are representative of two independent experiments (A, B: Experiment 1; C, D: Experiment 2). Scale bar = 500 nm.

Article Snippet: The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers): The human breast cancer cell line HCC70 (ATCC ®CRL 2315TM; Lot #58033362; RRID: CVCL_1270) was obtained from the American Type Culture Collection (ATCC; Manassas, VA).

Techniques: